Python 3 is required, and you should also install tkinter to run the GUI.

To run it from the script, simply call MgfRead.py with Python:

python3 MgfRead.py

You can also copy the function such as parse_msconvert to run it in your own script if you don't need GUI.

To build an exe which you can run later without Python, you should install PyInstaller first, and then run:

build_win.bat # for Windows

sh build_mac.sh # or, for macOS